刊物名（原文）：Biochemical

Engineering Journal

刊物名（中文）：生物化學(xué)工程學(xué)報

刊物國別：

發(fā)表時間（原文）：2015,101:44–54

發(fā)表時間（中文）：發(fā)表于2015年101期44-54頁

刊物類型（EI/SCI/SSCI），級別：SCI影響因子2.45分類二區(qū)

論文題目（原文）：Rhamnolipid production

from waste cooking oil using Pseudomonas SWP-4

論文題目（中文）：Pseudomonas SWP-4菌株轉(zhuǎn)化餐廚廢油生產(chǎn)鼠李糖脂

論文作者（原文）：Guihong Lan, Qiang Fan, Yongqiang Liu, Chao

Chen，Guixiang Li , Yu Liu, Xiaobo Yin

論文作者（中文）：蘭貴紅，范強，劉永強，陳超，李桂香，劉余，尹小波

論文摘要（原文）：The present work aims to produce rhamnolipid from waste cooking oil (WCO) using a newly isolated bacterium named Pseudomonas SWP-4. SWP-4 was a high-yield strain that could accumulate rhamnolipid steadily even in decline phase and gave a maximum rhamnolipid yield of 13.93 g/L and WCO utilization percent around 88%. The critical micelle concentration of the produced rhamnolipid was only 27 mg/L and its emulsification index against n-hexadecane reached around 59%. Moreover, it reduced the surface tension of water from 71.8 mN/m to 24.1 mN/m and the interfacial tension against n-hexadecane from 29.4 mN/m to 0.9 mN/m. Results of biosurfactant stability show the rhamnolipid was effective when the salinity was lower than 8% and pH value ranged from 4 to 10, and it was quite thermostable based on thermal gravity analysis. Furthermore, it maintained high surface activity even after incubation under extreme conditions i.e., pH of 4.0, salinity of 8% and temperature of 80 ?C for half a month. Based on free fatty acids metabolism analysis, Pseudomonas SWP-4 consumed palmitic acid, oleic acid and linoleic acid chiefly. All these characteristics demonstrate bioconversion and biodegradation of WCO by Pseudomonas SWP-4 is a promising and commercial way of rhamnolipid production and waste treatment.

論文摘要（中文）：本研究利用Pseudomonas SWP-4菌株轉(zhuǎn)化餐廚廢油生產(chǎn)鼠李糖脂，優(yōu)化后其最佳產(chǎn)率為13.93g/L，餐廚廢油的轉(zhuǎn)化率為88%；產(chǎn)物的臨界膠束濃度(CMC)為27mg/L,乳化n-十六烷乳化率為59%。對水體的表面張力從71.8mN/m降低至24.1mN/m。結(jié)果顯示產(chǎn)物有廣泛的耐鹽性，耐溫性，基本不受溫度、pH值與鹽濃度影響。

對應(yīng)開放實驗題名：生物轉(zhuǎn)化餐廚廢油產(chǎn)鼠李糖脂假單胞菌株的分離與代謝機理

對應(yīng)開放實驗編號：KSZ14094

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